Martin is generally correct, just to add to the above in the event you're printing digitally. Your digital press maybe have a calibration process which would be similar to the linearization mentioned above (or below, i forget how the thread works).
This process will measure your individual separations with tints from 0-100, usually in a random grid across the sheet, if you use a G7 method you'll see both your primaries and shades of grey across the sheet in a similar fashion.
Between this and your substrate calibrations, you will be accounting for dotgain.
In fact, the substrate calibrations should show a set of curves afterwards. one set being your target, and the other, what was measured.